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hcv- negative human hepatoma cell line huh7.5 cells  (Apath LLC)

 
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    Apath LLC hcv- negative human hepatoma cell line huh7.5 cells
    ( A <t>)</t> <t>Huh7.5</t> or Huh7.5-FL cells grown for 48 h were stained with antibodies against <t>HCV</t> proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.
    Hcv Negative Human Hepatoma Cell Line Huh7.5 Cells, supplied by Apath LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcv-+negative+human+hepatoma+cell+line+huh7%2E5+cells/pmc03464290-32-5-28?v=Apath+LLC
    Average 90 stars, based on 1 article reviews
    hcv- negative human hepatoma cell line huh7.5 cells - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Activation of the Connective Tissue Growth Factor (CTGF)-Transforming Growth Factor β 1 (TGF-β 1) Axis in Hepatitis C Virus-Expressing Hepatocytes"

    Article Title: Activation of the Connective Tissue Growth Factor (CTGF)-Transforming Growth Factor β 1 (TGF-β 1) Axis in Hepatitis C Virus-Expressing Hepatocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0046526

    ( A ) Huh7.5 or Huh7.5-FL cells grown for 48 h were stained with antibodies against HCV proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.
    Figure Legend Snippet: ( A ) Huh7.5 or Huh7.5-FL cells grown for 48 h were stained with antibodies against HCV proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.

    Techniques Used: Staining, Confocal Microscopy, Expressing, Western Blot, Transfection



    Similar Products

    hcv- negative human hepatoma cell line huh7.5 cells  (Apath LLC)
    90
    Apath LLC hcv- negative human hepatoma cell line huh7.5 cells
    ( A <t>)</t> <t>Huh7.5</t> or Huh7.5-FL cells grown for 48 h were stained with antibodies against <t>HCV</t> proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.
    Hcv Negative Human Hepatoma Cell Line Huh7.5 Cells, supplied by Apath LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcv-+negative+human+hepatoma+cell+line+huh7%2E5+cells/pmc03464290-32-5-28?v=Apath+LLC
    Average 90 stars, based on 1 article reviews
    hcv- negative human hepatoma cell line huh7.5 cells - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Huh7.5 or Huh7.5-FL cells grown for 48 h were stained with antibodies against HCV proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.

    Journal: PLoS ONE

    Article Title: Activation of the Connective Tissue Growth Factor (CTGF)-Transforming Growth Factor β 1 (TGF-β 1) Axis in Hepatitis C Virus-Expressing Hepatocytes

    doi: 10.1371/journal.pone.0046526

    Figure Lengend Snippet: ( A ) Huh7.5 or Huh7.5-FL cells grown for 48 h were stained with antibodies against HCV proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.

    Article Snippet: In this study we used HCV- negative human hepatoma cell line Huh7.5 cells and Huh7.5 cells harboring full genome length HCV [Con1/FL-Neo HCV1b FL (S2204I)] (Huh7.5-FL) replicon cells (Apath, LLC; St. Louis, MO) and propagated in complete Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA).

    Techniques: Staining, Confocal Microscopy, Expressing, Western Blot, Transfection